PHB1 mRNA在肝硬化及肝癌组织中的表达

Expression of PHB1 mRNA in hepatocellular carcinoma and cirrhosis

目的:探讨肝癌组织、肝硬化组织及正常对照组织中阻抑蛋白1(prohibitin1,PHB1)mRNA的表达及关系.方法:采用逆转录-聚合酶链反应方法检测36例肝细胞癌(hepatocellular carcinoma,HCC)患者肝癌组织、癌旁肝硬化组织、非肝癌的肝硬化患者活检组织、肝血管瘤及肝内胆管结等对照组组织中PHB1 mRNA的表达,用捷达801分析软件对结果进行相对定量分析.结果:肝癌组织、肝硬化组织对照组PHB1mRNA表达率分别为70%、84.1%、100%,表达率之间无明显统计学差异(P>0.05),肝癌组织、肝硬化组织和对照组PHB1 mRNA表达量分别为0.81±0.57、1.16±0.58、1.97±1.24;扩增产物表现出表达量的不同,呈现渐变趋势,肝癌组织、肝硬化组织与对照组之间明显统计学差异(P<0.05),HCC组织与LC组织比较无统计学差异(P>0.05).结论:PHB1参与了肝硬化、肝癌的发生发展,可能是肝硬化和肝癌潜在诊断标志物和治疗靶点.

AIM： To investigate the expression of prohibitin I （PHB1） mRNA in hepatocellular carcinoma and cirrhosis. METHODS： RT-PCR was used to detect the expres- sion of PHB1 mRNA in 36 specimens of hepatocel- lular carcinoma, cirrhosis, or normal liver tissues. RESULTS： The positive rate of PHB1 mRNA expression showed no significant difference be- tween hepatocellular carcinoma, cirrhosis and normal liver tissues （70%, 84.1% and 100% re- spectively; all P 〉 0.05）. The expression level of PHB1 mRNA was significantly lower in hepato- cellular carcinoma and cirrhosis than in normal liver tissues （0.81 ± 0.57, 1.16 ± 0.58 vs 1.97 ±1.24; both P 〈 0.05）.CONCLUSION： PHB1 may be involved in the de- velopment and progression of hepatoceUular carci- noma and cirrhosis, and it may be used as a poten- tial biomarker for diagnosis and target for therapy.