黄芪当归不同配伍对气虚血瘀证大鼠血液流变学及血清干扰素-γ、白细胞介素-4的影响

Effect of Compatibility of Radix Astragali and Radix Angelicae Sinensis in Different Proportion on Hemorheology and Contents of IL-4, IFN-γ in Blood of Qi Deficiency and Blood Stasis Rat Model

目的探讨黄芪当归配伍对气虚血瘀证大鼠血流动力学的改善及对Th1/Th2细胞的调节作用。方法采用限食+强迫游泳+皮下注射肾上腺素方法造成大鼠气虚血瘀证模型,分别用不同比例黄芪当归配伍灌胃给药治疗21 d。用血流变仪测定血液流变学指标,ELISA法测定血清中干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)含量。结果服药21 d后,与模型组比较,芪归1∶1组、芪归3∶1组、芪归5∶1组血流变低切指标明显减小,血黏度明显改善。模型组大鼠脾指数、胸腺指数均明显减小,而芪归5∶1组服药后脾指数明显上升。模型组IFN-γ降低,IL-4升高,以Th2细胞反应占优势;芪归3∶1组、芪归5∶1组能升高血清IFN-γ含量,芪归5∶1组能降低IL-4含量。结论黄芪当归配伍对气虚血瘀证大鼠模型具有改善血液流变及增强免疫作用,并且可以调节Th1/Th2细胞平衡。其中以黄芪比例大于当归时效果较好,综合作用芪归5∶1组最佳。

Objective To observe the effects of the compatibility of Radix Astragali and Radix Angelicae Sinensis in different proportion on hemodynamic and balance of Th1/Th2 cells of Qi deficiency and blood stasis rat model. Methods Qi deficiency and blood stasis rats were caused by restricted diet, forced swimming and norepinephrine subcutaneous injecting, and treated by compatibility of Radix Astragali and Radix Angelicae Sinensis in different proportion of 5 ： 1, 3 ： 1, 1 ： 1 and 1 ： 2 for 21 days. The indexes of hemorheology were detected with hemorheological analyser, and the level of IFN-γ and IL-4 in serum were tested by ELISA. Results Compared with model group, the compatibility of Radix Astragali and Radix Angelicae Sinensis in proportion of 1 ： 1, 3 ： 1 and 5 ： 1 groups reduced the low shear blood viscosity. The spleen index＇of model group decreased, and compatibility of Radix Astragali and Radix Angelicae Sinensis in 5 ： 1 proportion group increased spleen index. The level of IL-4 increased and IFN-γ decreased in the serum of model group, the compatibility of Radix Astragali and Radix Angelicae Sinensis in proportion of 3 ： 1 group increased the level of IFN-γ. Astragalus angelica 5 ： 1 group decreased the level of IL-4 and increased the level of IFN-γ. Conclusion The compatibility of Radix Astragali and Radix Angelicae Sinensis can improve hemorheology, adjust the balance of Thl/Th2 cells of Qi deficiency and blood stasis rats. The effects were better when Radix Astragali＇s dosage greater than that of Radix Angelicae Sinensis, and the group of 5 ： 1 proportion was the best.