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白玉菇多糖提取方法的比较和优化 被引量:13

Comparison and Optimization Among Extraction Technologies of Polysaccharide From Pleurotus Nebrodensis
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摘要 优选白玉菇多糖的提取工艺.采用热水浸提法、超声波辅助提取法、微波辅助提取法和碱提法4种方法提取白玉菇多糖,并采用响应面法优化超声波辅助提取法.热水浸提法、超声波辅助提取法、微波辅助提取法和碱提法提取多糖得率分别为2.90%、5.48%、4.86%、4.71%.超声波辅助提取法提取白玉菇多糖的得率与其他3种方法比较有显著差异.响应面法优化超声波辅助提取法的最优条件为:液料比23∶1,超声时间28 min(300 W),在92℃下热水浸提2 h,重复3次,测定白玉菇多糖的得率为6.03%.超声波辅助提取法可以显著提高白玉菇多糖得率. The purpose of the experiment is to choose the best extraction technology of polysaccharide from Pleurotus ne- brodensis. The Pleurotus nebrodensis was used as the raw material for extracting polysaccharide by means of hot water ex- traction, uhrasonic-assisted extraction, microwave-assisted extraction, ulkali mention. A central composite design followed by response surface analysis was carried out to optimize the ultrasonic-assisted extraction parameters. The yields of the four extraction technologies were 2.9% , 5.48% , 4.86% and 4.71%. The results of determination of polysaccharide content showed that ultrasonic-assisted extraction had significant differences in the four extraction technologies. The optimal ultrasonic-assisted extraction conditions were 28 rain at ultrasonic power 300 W with a water-material ratio of 23:1 and three times repeated extractions at 92 ℃ for 2 h each time,which yielded an extraction yield of 6.03%. The ultrasonic-assisted extraction could significantly imnrove the yield of nolvsaccharide from Pleurotus nebrodensis.
出处 《南京师大学报(自然科学版)》 CAS CSCD 北大核心 2013年第3期87-92,共6页 Journal of Nanjing Normal University(Natural Science Edition)
基金 江苏省高校自然科学基础研究项目(10KJD550003) 江苏省普通高校研究生科研创新计划项目(CXLX11_0895)
关键词 白玉菇 多糖提取 超声波 微波 碱提 响应面 Pleurotus nebrodensis, polysaccharides extraction, ultrasound, microwave, ulkali mention, responsesurface methodology
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