酶促木质素与酚聚合反应分子量及分布实验

Study on molecular weight controlling and distribution in copolymerization of lignin and phenol with peroxidase as catalyst

在反相微乳液系统中 ,进行了过氧化物酶催化木质素与酚共聚反应实验 ,考察了酶浓度、WO(水 /表面活性剂 ,质量比 )、表面活性剂浓度、单体 (酚、木质素 )浓度及醇烃比对聚合物分子量的影响。结果表明 ,聚合物重均分子量可用表面活性剂浓度、酶浓度、单体浓度及油相中醇烃比来调控。用单纯形优化法关联了实验数据 ,得到了聚合物重均分子量的关联式 :M =6 2 7× 10 4 (Y1× 10 7) -0 .3 4Y0 .982 Y0 .83 Y2 .4 64 Y1.0 55。聚合物分子量分布出现双峰 ,是两种反应机理竞争的结果。

The copolymerization of lignin and phenol with horseradish peroxidase as catalyst in reversed micellar system was researched.The influence of concentration of enzyme, surfactant and monomer(phenol,lignin),mass ratio of water and surfactant ( WO ), alcohol and hydrocarbon on the molecular weight of copolymer was investigated. The results showed that the mean molecular weight could be adjusted and controlled by concentration of surfactant, enzyme, monomer, and ratio of alcohol and hydrocarbon in organic solvent phase. The data was related by simplex optimization method, and the related formula of mean molecular weight was obtained as M=6 27×10 4(Y 1×10 7) -0 34 Y 0 98 2Y 0 8 3Y 2 46 4Y 1 05 5 The result of competition of two reaction mechanism led to two peaks of copolymer molecular weight distribution.