分光光度法测定溪黄草及其近缘种中总黄酮含量

Determination of total flavones from Rabdosia lophanthoides and its relative species by spectrophotometry

采用微波辅助萃取法提取溪黄草及其近缘种中的黄酮类化合物,以芦丁为对照品,以亚硝酸钠-硝酸铝-氢氧化钠作为显色体系,用可见分光光度法测定了溪黄草及其近缘种中总黄酮的含量.该方法在0.00～1.25 mg范围内吸光度与含量呈良好的线性关系,加标回收率为97.43 ％～103.0％,相对标准偏差（n=6）为1.83％.结果表明：溪黄草及其近缘种中总黄酮含量差异不大.该方法操作简便、结果稳定、重复性好,可用于溪黄草及其近缘种中总黄酮含量检测,实验结果为溪黄草及其近缘种的开发和利用奠定了基础.

Flavonoids were extracted from Rabdosia lophanthoides and its relative species by microwave-assisted method. Al （NO）3-NaNO2-NaOH colorimetric method was applied to determine the total flavonoids by spectrophotometry with rutin as the standard. The content of the standard was well correlated to its optical density in the range of 0.00-1.25 mg. And the addition standard recoveries were in the ranges of 97.43 %-103.0 %, the standard deviation （n=6） of measurement was 1.83%. The method was proved to have the advantages of simple operation, stability and good repeatability, and it would be helpful for determining the total flavonoids of Rabdosia lophanthoides and its relative species. The result provided scientific basis for exploiting Rabclosia lophanthoides and its relative species.