海水浸泡与低温对腹部开放伤大鼠血清VEGF含量的影响

Influence of seawater immersion and hypothermia on vascular endothelial growth factor concentrations in the serum of rats with open abdominal injury

目的研究海水浸泡与低温对腹部开放伤大鼠血清中血管内皮生长因子(VEGF)含量的影响。方法将SD大鼠随机分为腹部开放伤合并常温生理盐水浸泡组(对照组)、腹部开放伤合并低温生理盐水浸泡组(低温组)、腹部开放伤合并常温海水浸泡组(常温海水组)和腹部开放伤合并低温海水浸泡组(低温海水组),各组浸泡1 h后,分别检测实验大鼠血清中VEGF含量。结果海水浸泡、低温及其两者的交互作用对大鼠血清VEGF含量的影响均有统计学意义(P<0.05或P<0.01)。结论海水浸泡可引起大鼠血清VEGF含量升高,低温不能独立促使血清VEGF含量升高,但是可以加重海水浸泡引起的血清VEGF含量升高。

Objective To study the influence of seawater immersion and hypothermia on vascular endothelial growth factor （VEGF） concentrations in the serum of rats with open abdominal injury. Methods SD rats were randomly divided into the following four groups： group 1 was open abdominal injury and normal saline immersion（ control group）, group 2 was open abdominal injury and normal saline immersion and hypothermia （hypothermia group ） , group 3 was open abdominal injury and seawater immersion （normal seawater temperature group） , and group 4 was open abdominal injury and seawater immersion and hypothermia（ low seawater tempera- ture group）. After 1 hour, VEGF concentrations in the serum of all the rats were detected by ELISA. Results The main influence of seawater immersion, hypothermia and their interaction on VEGF concentration had statistical significance （ P 〈 0.05 or P 〈 0.01 ）. Conclusion Seawater immersion correlates with high level VEGF concentration in the serum of the rats. Although hypothermia does not raise VEGF concentration alone,it can facilitate the influence of seawater immersion on VEGF concentration.