不同鹅品种脂联素基因第2外显子多态性分析

Polymorphism analysis on exon 2 of adiponetin gene in different breeds of geese

为了寻找中国部分地方白鹅品种脂联素基因在外显子上可能存在的点突变,并检测其在不同鹅品种中分布情况及分析群体遗传参数。本试验以5个中国地方白鹅品种为试验材料,在脂联素基因外显子2上设计2对引物,利用PCR-SSCP方法寻找点突变,并分析基因型在不同鹅群体中的分布情况和群体遗传参数。结果显示,在外显子第351处发生了A→G突变,但未引起氨基酸序列发生变化。豁眼鹅X和Y等位基因频率最为接近,分别为0.566 7和0.433 3,其他4个鹅品种均以Y等位基因频率显著高于X等位基因频率。豁眼鹅基因型分布与其他鹅群体存在极显著差异(P<0.01);浙东白鹅基因型分布与太湖鹅、四川白鹅存在显著差异(P<0.05)。除浙东白鹅为低度多态外,其他鹅品种为中度多态。表明鹅脂联素基因在不同品种中具有较丰富的多态性,可以为鹅品种育种和保种提供依据。

To search single nucleotide polymorphisrns （SNPs） in exon of adiponectin gene in domestic goose, and to i- dentify SNP location in goose breeds, then to analyze genetic parameter of goose populations, two pairs of primers were de- signed to detect SNPs of exon 2 on adiponetin gene in five breeds of goose by polymerase chain reaction-single-strand confor- mation polymorphism（PCR-SSCP）. The results showed that one SNP （A--G） was found in the 351 bp of exon 2, but it didn＇ t cause the change of amino acid sequence. The allele frequencies for X and Y of Huoyan goose were the closest, being O. 566 7and O. 433 3, respectively. However, the frequencies of allele Y were significantly higher than those of allele X in oth- er four goose breeds. The genotypes of Huoyan goose differed significantly from those of other goose breeds （P〈0. 01 ）. The genotypes of Zhedong white goose was significantly different from Taihu goose and Sichuan white goose （P〈0. 05）. Except for Zhedong white goose, the polymorphism information contents （PICs） of other breeds were moderate. The adiponectin gene, due to its high polymorphism in goose breeds, could be emoloved as a candidate gene in goose breeding.