摘要
目的:探讨耐胆盐革兰阴性菌检查中,胰酪大豆胨肉汤预培养是否能达到对菌体只复苏不增殖的目的;肠道增菌肉汤颜色指示特性的必要性。方法:采用不同菌株和不同厂家培养基,依据《美国药典》35版方法进行对比考查。结果:胰酪大豆胨肉汤按规定的最短预培养时间培养后菌体有增殖;不同厂家肠道增菌肉汤培养基的颜色指示特性具有不一致性。结论:微生物经胰酪大豆胨肉汤预培养在复苏的同时菌数有所增加;肠道菌增菌肉汤无需观察颜色指示特性。
Objective ： To find whether TSB could make bile - tolerant Gram - ngative bacteria proliferate and EEB could serve as a specific indicator. Methods ： According to the method provided in USP 35, various bacteria and cultures were used for the con- trast tests. Results ： Bacteria proliferation was found in the required minimum pre - incubation time with TSB for the selected bacteria and cultures. The EEBs from different factories exhibit different colors in the different cultures. Conclusion： The pre -incubation using TSB does not only resuscitate the bacteria but also proliferate the organisms at the same time. The EEB can not be used for an indicator.
出处
《天津药学》
2013年第5期9-12,共4页
Tianjin Pharmacy
