朱砂七总蒽醌对S_(180)荷瘤小鼠凋亡基因表达的影响

Effect of total anthraquinone from Zhushaqi on expression of apoptosis gene in S_(180)-bearing mice

[目的]观察朱砂七总蒽醌对S180荷瘤小鼠凋亡基因c-myc、Bcl-2和突变型p53表达的影响。[方法]建立S180荷瘤小鼠模型,分成4组:模型对照组,阳性对照环磷酰胺组和朱砂七总蒽醌大、小剂量组,灌胃给药第11天,取各组小鼠的瘤组织,采用实时定量PCR法检测各组小鼠肿瘤组织c-myc、Bcl-2和突变型p53基因的表达。[结果]朱砂七总蒽醌1.2 g/kg和0.3 g/kg剂量组c-myc、Bcl-2和突变型p53mRNA表达量较模型组均显著降低,与模型组比较有统计学意义(P<0.01或P<0.05)。[结论]朱砂七总蒽醌对S180荷瘤小鼠的抑瘤作用可能是通过下调小鼠S180瘤组织c-myc、Bcl-2和突变型p53mRNA的表达而实现的。

[Objective] To study the effect of total anthraquinone from Zhushaqi（ZSQ） on c-myc, Bcl-2 and p53 mRNA expression in S180 tumour-bearing mice. [Methods] The S180 mice model was established. The mice were divided into 4 groups： blank control, positive control with eyclophosphamide and ZSQ in high or low dose. Ten days after continuous treatment, all the mice were sacrificed and the tumor tissue was collected. Real-time PCR was used to determine the effect of ZSQ on the expression of c-myc, Bcl-2 and p53 mRNA. [Restilts] ZSQ could significantly reduce the expression of c-myc, Bcl-2 and p53 mRNA expression in S180 tumor tissue. The average levels of c-myc, Bcl-2 and p53 expression in low-dose and high-dose groups of ZSQ had significantly difference to that in control group （P〈0.01 or P〈0.05）. [Conclusion] Down-regulating the c-myc, Bcl-2 and p53 mRNA expression is probably one of the antitumor mechanisms of ZSQ.