摘要
目的提供一种既简单又实用的方法,用来鉴别染色南五味子劣质品。方法:采用70%乙醇浸泡,薄层色谱用胭脂红、赤藓红和酸性红73为对照试剂,乙酸乙酯-正丁醇-乙醇-氨水-水(1∶3∶3∶1∶1)为展开剂,可见光下检视。结果:染色南五味子可通过醇液是否迅速被染红来区分;薄层色谱法专属性强、分离度、重现性较好,并用标准规定方法进行了佐证。结论:该方法实用易行,可用于鉴别染色南五味子。
Objective a simple method is provided to discriminated the Badness Product of staining Kadsura. Method soaked with 70% Ethanol. thin - layer chromatography scanning(TLCS) was performed ; cochineal, erythrosine,aeid red 73 were used as Control agents ,Ethyl Acetate- butanol -Ethanol- ammonia- water( 1: 3: 3: 1: 1 )were used as the developer, detected under visible light. Result staining Kadsura were discriminated by rapid incarnadine of Aqueous Alcohol Solutions, TLC is good in Specialization,separating degree ,repeatability and verified with standard regulation methods. Conclusion This method is easy to operate and could be used to identify staining Kadsura.
出处
《湖北中医药大学学报》
2013年第5期38-40,共3页
Journal of Hubei University of Chinese Medicine
关键词
染色南五味子
鉴别
薄层色谱
高效液相色谱
Kadsura ,discrimination, thin layer chromatography, high performance liquid chromatography