聚球藻7002基因组DNA提取方法比较

Comparison of Different Methods for Extracting Genomic DNA of Synechococcus sp. PCC 7002

以聚球藻7002(Synechococcus sp.PCC 7002)为试材,采用高盐法、CTAB法、PVP法和石英砂法4种方法对其基因组DNA进行提取,并使用核酸测定仪检测提取到的基因组DNA的纯度和浓度,对基因组DNA进行琼脂糖凝胶电泳分析及PCR扩增检测。结果表明:4种方法均能提取到符合分子生物学要求的聚球藻7002基因组DNA,其中PVP法纯度最高,高盐法、石英砂法次之,CTAB法纯度最低;石英砂法浓度最高,PVP法、高盐法稍低,CTAB法浓度最低。4种方法提取到的基因组DNA均能扩增出理想条带。

Taking Synechococcus sp. PCC 7002 as material, four methods of high-salt method, CTAB method, PVP method and quartz sand method were used to extract the genomic DNA of it, and the concentration and purity of DNA were detected by nucleic acid analyzer, agarose condensate gel electrophoresis and PCR amplification were analyzed. The results showed that the four methods were able to extract genomic DNA from Synechococcus sp. PCC 7002,the DNA which was extracted by PVP method had the highest purity, containing less protein and RNA,the high-salt method and the quartz sand method followed, the CTAB method had the lowest purity. The I）NA which was extracted by the quartz sand method had the highest concentration, the PVP method followed, then the high-sah method, the CTAB method had the minimum concentration. The genomic DNA which was extracted by four kinds of method could amplify the ideal band.