万寿菊根尖染色体观测方法的优化

Optimization of Microscope Observation Method on Root-tip Chromosomes of Marigold

以万寿菊‘QU2—2’为试材，从取材时间、根的长度、预处理方法、解离时间、染色时间、压片工具6个方面对根尖染色体制片方法进行了筛选和优化。结果表明：万寿菊根尖染色体观测采用室内催根培养，于上午9：14，剪取根长1．0cm的根尖，利用低温（0～4℃）水浴进行预处理24h、1．0mol／L盐酸解离7min、染色25min、结合带橡皮头铅笔压片，可以获得高质量的有丝分裂前中期临时片。

Taking the root-tip of Tagetes erecta L. ＇ QU2-2＇ as the experimental material, the material taking time, length of the root, material pretreatment method, dissociating time, dyeing time and squashing tools were screened and optimized. The resuhs showed that using indoor root culture,observed the high-quality images of the chromosomes of root-tips at 9： 14,1.0 cm of the root length,the cool water pretreatment （0-4℃）, 1.0 mol/L HCl for dissociation in 7 minutes, dyeing in 2,5 minutes,squashing the slides with a rubber head pencil,could observe clear milotic prometaphase.