西芹种子浸提液对黄瓜枯萎病菌的化感作用机理研究

Study on Allelopathy Mechanism of the Extract Solution of Parsley Seeds on Fusarium oxysporium f.sp. cucumeris

以美国西芹种子为试材,采用孢子萌发法和液体培养法,研究了西芹种子浸提液对黄瓜枯萎病菌菌丝生长、孢子形成和孢子萌发的影响,以探讨其化感作用机理。结果表明:经西芹种子各浸提液化感作用后,黄瓜枯萎病菌菌丝变细,分叉少;孢子形成、孢子萌发均受到不同程度的化感抑制作用,孢子形成抑制率分别为40.32%、62.26%、60.42%,孢子萌发抑制率分别为19.04%、27.28%、28.08%。在电镜下观察各处理病菌孢子膜结构遭到破坏,胞内结构紊乱,内含物外渗。

Taking American parsley seeds as test material,the effect of the extracts solution of parsley seeds on the mycelium growth,spore formation and spore germination of Fusariurn oxysporium f. sp. cucumeris were studied using spore germination and spore suspension liquid method,to discuss the allelopathy mechanism. The results showed that the mycelium of Fusarium oxysporium f. sp. cucumerinum was thinner and less forked after treated with three parsley seeds extraction （distilled water, ethanol and acetone）. The spore formation and spore germination were allelopathic inhibited differently after treated,the inhibition rates of spore formation were 40. 32% 62. 26 % 60. 42%, and the inhibition rates of spore germination were 19. 04%, 27. 28%, 28. 08%. Through the electron microscopy observation, the structure of membranes of Fusarium oxysporiwn f. sp. cucumeris, spores were destructed by three parsley seeds extraction treatment, the intracellular structural was disorded and the contentsexosmosed.