枳椇总皂苷提取工艺研究

Study on the Extraction Technology of Total Saponins fromHovenia dulcis

以枳椇为试材,采用酸碱泡沫法、α?萘酚-硫酸沉淀法、乙酐-硫酸显色法对枳椇的各部分(包括叶片、枝干、果梗、果渣、种子、种壳)总皂苷含量进行定性分析,用重量法和溴量法进行定量分析,按正交实验设计,研究了提取温度、料液比和提取时间对总皂苷的提取率的最佳工艺。结果表明:枳椇果梗中总皂苷含量最高。最优的提取工艺是纯水提取,提取温度60℃,料液比1∶15mg/L,提取时间4h(回流1次,每次2h),枳椇果梗中总皂苷提取量为16.5mg/g。

Taking Hovenia dulcis as material, using acid-base foam method, a-naphthol-sulfuric acid precipitation method, acetic anhydride and sulfuric acid method for qualitative analysis of total saponin content, weight method and bromine method for quantitative analysis in each part of the Hovenia dulcis （including the leaves, branches, fruit, fruit residue, seed,shell）, according to the total saponin extracting rate, the extracting techniques, including extracting temperature, solid to liquid ratio, extracting time were optimized by orthogonal design. The results showed that the total saponin content in stems was the highest. The sample was extracted by pure water,the extracting temperature was 60℃,the ratio of solid-solution was 1 ： 15 mg/L,the extracting time was 4. 0 h（refluxing extraction for 1 times,2 h for each time）,and the total extraction rate of Hovenia dulcis fruit was 16. 5 mg/g.