清热化痰祛瘀方对高脂血症大鼠肝SR-BImRNA及蛋白表达的影响

Effect of “Qingre Huatan Quyu Formula”on mRNA and protein expression of SR-BI in rats with hyperlipidemia

目的研究清热化痰祛瘀方对高脂血症大鼠肝脏高密度脂蛋白受体(SR-BI)mRNA及蛋白表达的影响。方法将SD大鼠随机分为正常组、模型组及清热化痰祛瘀方小、中、大剂量组和阳性药对照组(立普妥组)。除正常组外,其余大鼠采用脂肪乳剂灌胃的方法建立大鼠高脂血症模型,各组给予相应的干预措施。治疗6周后,采用RT-PCR和Western Blot方法检测大鼠肝组织中SR-BI mRNA和蛋白表达的水平。结果与正常组比较,模型组大鼠肝组织中SR-BI mRNA和蛋白表达明显下调(P<0.01)。与模型组比较,清热化痰祛瘀方中、大剂量组大鼠肝组织中SR-BI mRNA表达和蛋白水平升高(P<0.05,P<0.01),立普妥组SR-BI mRNA表达和蛋白水平亦明显上调(P<0.05)。中、大剂量组上调SR-BI mRNA的表达优于小剂量组(P<0.05)。清热化痰祛瘀方大剂量组上调SR-BI mRNA表达的水平优于立普妥组(P<0.05)。结论清热化痰祛瘀方调脂、抗动脉粥样硬化的作用可能与其调控SR-BI mRNA和蛋白表达有关。

Objective To observe the clinical efficacy of ＂Qingre Huatan Quyu Formula＂ on mRNA and protein expression of SR-BI in rats with hyperlipidemia. Methods The SD rats were randomized into normal group, model group, ＂Qingre Huatan Quyu Formula＂ groups （large, medimn and small dose） , and positive control group. Except for normal group, rats in the other groups were intervened by corresponding methods. After 6 weeks, the mRNA and protein expression of SR-BI in rat＇s liver were examined by real-time PCR or Western Blot. Results Compared with normal group, the mRNA and protein expression of SR-BI in model group were reduced significantly（P 〈 0.01 ）. Compared with model group, the mRNA and protein expression of SR-BI in medium and large dose ＇＂ Qingre Huatan Quyu Formula＂ groups were increased markedly （ P 〈 0.05, P 〈 0.01 ） , and similar result was observed in Lipitor group （ P 〈 0.05）. The mRNA expression of SR-BI in medium and high dose ＂ Qingre Huatan Quyu Formula＂ groups were higher than that of the small dose group （P 〈 0.05 ）. The mRNA expression of SR-BI in large dose ＂ Qingre Huatan Quyu Formula＂ group was higher than that of the Lipitor group （ P 〈 O. 05 ）. Conclusion ＂Qingre ttuatan Quyu Formula＂ can regulate lipid and resist atherosclerosis, its mechanism may be association with regulating and controlling mRNA and protein expression of SR-BI