响应面法优化阿维菌素B_(1a)生产菌株复合发酵培养基

Optimization of Avermectin Complex Production Medium by Response Surface Methodology

以棉籽饼粉替代酵母粉,采用响应面法优化阿维链霉菌Streptomyces avermitilis生产菌株的工业复合发酵培养基。首先采用Plackett-Burman设计筛选出玉米淀粉、棉籽饼粉和豆粕粉3个重要影响因子,并利用响应面中心组合设计进一步优化,得阿维菌素B1a复合发酵培养基的最佳配方:玉米淀粉14.22%,棉籽饼粉1.19%,豆粕粉2.67%,钼酸钠0.002 2%,氯化钴0.02%,硫酸锰0.000 2%,硫酸铵0.05%。在上述条件下,阿维菌素B1a摇瓶发酵效价为5 235 IU/ml,比优化前(4 580 IU/ml)提高了14.3%。

With cotton seed powder as replacement for yeast powder, the industrial composite fermentation medium of avermectin B1a production was optimized by response surface methodology. Firstly, Plackett-Burman design was used to evalute the influences of the seven related factors in the original fermentation medium. It was found that corn starch, cotton seed powder and soya bean meal had a strong impact on avermectin Bla production. Using statistical methods （CCD） to further optimize the fermentation medium, a new avermectin Bla production medium was obtained as follows： corn starch 14.22%, cottonseed meal 1.19%, soya bean meal 2.67%, sodium molybdate 0.002 2%, cobalt chloride 0.02%, manganese sulfate 0.000 2% and ammonium sulfate 0.05%. Under the optimum conditions, the avermectin Bla titer of 5 235 IU/ml was obtained in shake flask, which was 14.3 % higher than the control （4 580 IU/ml）.