茉莉花叶色素的体外抗氧化活性研究

Study on Antioxidant Effect of Pigments in the Jasmine Leaves

目的对茉莉花叶色素的抗氧化作用进行研究。方法以蒸馏水为溶剂,从茉莉花叶中提取色素;采用比色法测定茉莉花叶色素对DPPH·、O2-·和·OH三种自由基的清除作用以及还原能力。结果茉莉花叶色素对三种自由基都有明显的清除作用,清除能力为DPPH·>O2-·>·OH;对Fe3+也具有较强的还原能力。结论清除率与浓度存在明显的量效关系,当色素浓度为3.5 mg/ml时,其清除率O2-·为49.3%、DPPH·为55.1%、·OH为20.9%;还原Fe3+能力随色素的浓度增加而增强。

Objective To study the antioxidant effect of the pigments in the jasmine leaves. Methods Using the distilled water as the solvent to extract pigments from the jasmine leaves ; It will determine the scavenging process of pigments in the jasmine leaves acting on DPPH ·, O2 -2 and · OH with the spectrophotometry and the reduction of Fe3 ＋ ability. Results Total pigments from jasmine leaves have significant scavenging effect to three kinds of free radicals, the result of scavenging process is that DPPH ·〉 O2 - · 〉· OH ; moreover,they also have strong ability of reduction to Fe3＋. Conclusion Clearance and concentration obvious concentration - response relationship. When the content of the total pigments of the jasmine leaves was p （Pigments） = 3.5 mg/ ml,the clearance rate of DPPH · , O2 - oand ·OH was 49.3% , 55.1% and 20.9%. The reduction ability of Fe3＋ to the pig- ments was enhanced when there concentration was increasing.