脾虚哮喘模型大鼠肺组织MUC5AC蛋白表达变化的研究

Expression change of MUC5AC in the rat asthmatic models with spleen-deficiency

目的观察脾虚对哮喘模型大鼠肺组织MUC5AC蛋白表达水平变化的影响,从而为"脾为生痰之源,肺为储痰之器"提供现代生物学基础。方法 SPF级Wistar大鼠36只,按照随机数字法分成3组,每组12只:对照组、脾虚哮喘组、哮喘组。采用复合因素建立脾虚模型,然后采用卵蛋白致敏和激发建立哮喘模型,测定外周血中EOS百分比;采用ELISA测定大鼠血清IL-4和IFN-γ的含量及支气管肺泡灌洗液中MUC5AC的含量;采用免疫组化测定大鼠肺组织MUC5AC的表达。结果与对照组比较,脾虚哮喘组和哮喘组外周血中EOS百分比均显著升高(P<0.01)。和哮喘组相比较,脾虚哮喘组外周血中EOS百分比显著升高(P<0.01)。与对照组比较,脾虚哮喘组和哮喘组血清中IL-4含量显著升高,血清中IFN-γ含量显著降低(P<0.01);与哮喘组相比较,脾虚哮喘组血清中IL-4含量显著升高(P<0.01),IFN-γ含量显著降低(P<0.01)。与对照组比较,脾虚哮喘组和哮喘组大鼠BALF中MUC5AC含量和肺组织MUC5AC的蛋白表达水平均显著升高(P<0.01)。与哮喘组相比较,脾虚哮喘组BALF中MUC5AC含量和肺组织MUC5AC的蛋白表达水平显著升高(P<0.01)。结论脾虚能够加重哮喘气道炎症和气道黏液高分泌。

Objective To investigate the effect and mechanism of invigorating the spleen to benefit qi prescriptions on airway mu- cus hypersecretion. Methods 36 male Wistar rats were divided into 3 groups randomly： control, spleen - deficiency asthmatic model ,asthmatic model. MUC5AC content were detected in bronchia alveolus lavage fluid （BALF） were measured by ELISA. Ex- pression of MUC5AC was detected by immunohistochemistry. Results The result from MUC5AC content and expressions of MUCSAC showed that compared with the control group, the two items were increased obviously in both spleen deficiency asthma and asthma group （ P 〈 0.01 ） . The MUCSAC protein expression level in spleen - deficiency group was higher than that of asthma group （ P 〈0.01 ） . Conclusion The spleen deficiency may increase expression of MUC5AC in lung in asthmatic rat models.