麦芽糖假丝酵母菌降解苯酚和间甲酚的特性研究

Biodegradation of Phenol and m-Cresol by Candida maltosa

考察了麦芽糖假丝酵母茵对单底物间甲酚的降解特性，并研究双底物降解体系中苯酚与间甲酚的相互作用。结果表明，麦芽糖假丝酵母菌能够以间甲酚为唯一碳源和能源，能在66h内降解240mg／L间甲酚，但该菌株降解间甲酚能力低于降解苯酚的。在双底物体系中，低浓度苯酚能够促进间甲酚降解，当苯酚浓度为200～400mg／L时，240mg／L间甲酚的降解速率得到提高；然而高浓度的苯酚对间甲酚的降解有抑制作用。间甲酚对苯酚的降解也存在抑制作用，且间甲酚对苯酚降解的抑制作用大于苯酚对间甲酚的。采用Abuhamed等人提出的方程能够准确描述双底物体系中菌株的生长动力学。

Biodegradation characteristics of single substrate m-cresol by Candida maltosa were investigated, and the interaction between phenol and m-cresol in the dual substrate biodegradation system was studied. The results showed that strain Candida maltosa could utilize m-cresol as sole carbon and en- ergy source. The strain could degrade 240 mg/L m-cresol in 66 h. The capacity to degrade m-cresol was obviously lower than that to degrade phenol. The low-concentration phenol enhanced the biodegradation of m-cresol in the dual substrate degradation system. 240 mg/L m-cresol could be completely degraded within a shorter period of time than m-cresol alone in the presence of 200 to 400 mg/L phenol. The high- concentration phenol inhibited the biodegradation of m-cresol ; on the other hand, m-cresol also inhibited the biodegradation of phenol, and the inhibitory effect of m-cresol was higher than that of phenol. The kinetics equation proposed by Abuhamed could adequately simulate cell growth in the dual substrate biodeg- radation system.