反义寡核苷酸抑制柯萨奇病毒B_3致病作用的实验研究

Experimental study on the inhibitory effect on coxsackievirus B3pathogenicity by antisense oligodeoxynucleotide

目的 :观察在细胞培养系统和小鼠体内反义寡核苷酸 (AODN)抑制柯萨奇病毒B3(CVB3)致病作用。方法 :观察细胞病变效应 (CPE) ,常规微量法滴定病毒的50 %组织细胞感染量 (TCID50)以及观察感染小鼠心肌组织的病理改变。结果 :1 AODN可推迟和减轻CVB3 感染细胞CPE ,且随AODN浓度增高 ,对CPE的抑制作用增强 ;培养上清中病毒滴度随AODN浓度升高而降低。2 随AODN剂量增加 ,感染小鼠心肌组织病毒滴度逐渐降低 ;心肌细胞病变程度逐渐减轻 ;心肌炎发病率也逐渐降低。结论 :AODN可能通过抑制CVB3 复制来保护Vero细胞和小鼠心肌组织抵抗感染。

Objective: To investigate the inhibitory effect of antisense oligodeoxynucleotide(AODN)as a specific inhibitor of CVB 3 replication on CVB3 pathogenicity in vero cells and in Balb/C mice. Methods: Cytopathic effect(CPE)was investigated,50% tissue culture infection dose(TCID50)was titrated on vero cells monolayer and cardiac histological lesions were investigated. Results:The specific AODN could significantly inhibit CPE of CVB3_infected vero cells and decrease viral titres in culture supernatant in dose dependent manner.The specific AODN could also significantly decrease viral titres of myocardial tissue,lighten cardiac lesions and reduce incidence rate of myocarditis in mice in dose dependent manner. Conclusion: AODN may be protect vero cells and myocardial tissue against CVB3 infection by inhibiting CVB3 replication.