摘要
[目的]建立低成本简单、而灵敏度高、准确性好的检测汞离子新方法。[方法]利用分光光度计扫描加入汞离子前后胶体金-DNA体系光谱,通过比较加入汞离子前后光密度比值(D660nm/D530nm)变化大小来快速定量汞离子。[结果]方法的线性范围为0.1～2.0μg/L,线性相关系数r=0.995,检出限为0.2μg/kg。[结论]胶体金-DNA体系法测定食品中汞离子简便、灵敏。
[ Objective ] To establish a simple and rapid method for mercury ion detection. [ Methods ] Variations of D660nm/D530nm before and after adding mercury ion were compared through the spectroscopy of coUoidal gold and DNA system which was scanned with a spectrophotometer to determine the content of mercury ion. [ Results ] The linear range of this method was 0.1-2.0 μg/L, the correlation coefficient （r） was 0.995, and the detection limit was 0.2 μg/kg. [ Conclusion ] The method based on colloidal gold and DNA system is simple and rapid for mercury ion detection in food.
出处
《环境与职业医学》
CAS
北大核心
2013年第10期800-801,共2页
Journal of Environmental and Occupational Medicine
基金
江苏省预防医学科研立项指导项目(编号:YZ201007)
江苏省南通市科技局社会发展计划(编号:HS2011027)
关键词
胶体金
DNA
汞离子
colloidal gold
DNA
mercury ion
