中华眼镜蛇毒组分联合活化免疫细胞对白血病KG1a细胞的杀伤作用

Cytotoxicity of Naja Naja Actra Venom Component Combined with Activated Immune cells on Leukemia Cell Line KG1a

本研究旨在探讨中华眼镜蛇毒组分(Naja Naja Actra Venom Component,NNAVC)与活化免疫细胞联合对人急性髓系白血病系KG1a细胞的杀伤作用。采用CCK-8法检测不同浓度NNAVC对KG1a细胞的杀伤作用,乳酸脱氢酶(LDH)检测试剂盒测定活化免疫细胞对KG1a细胞的细胞毒作用、NNAVC与活化免疫细胞联合杀伤KG1a细胞以及NNAVC作用后存活的KG1a细胞对活化免疫细胞的杀伤敏感性变化。结果显示,NNAVC对KG1a细胞的抑制率随药物浓度的增加而增高,而活化免疫细胞对KG1a细胞的杀伤活性随效靶比的升高(6.25∶1、12.5∶1和25∶1)分别达到12.30%、24.85%和52.26%。NNAVC与不同效靶比(10∶1、20∶1)的活化免疫细胞联合,对KG1a细胞的杀伤率达到56.21%和85.59%,高于两因素各自的单独作用效果。以不同浓度NNAVC杀伤后存活的KG1a细胞为靶细胞,活化免疫细胞对其杀伤活性在效靶比为10∶1时分别为25.65%、31.33%、28.63%和16.78%,在效靶比为20∶1时分别为40.62%、44.70%、44.62%、40.72%。结论:NNAVC与活化免疫细胞在体外均可以有效杀伤KG1a细胞,二者联合应用能够增强对KG1a细胞的杀伤作用。

This study was aimed to investigate the cytotoxic effect of the Naja Naja Actra Venom Component （NNAVC） combined with activated immune ceils on human acute myeloblastic leukemia line KG1 a ceils. The cytotoxic effects of NNAVC at different concentrations on KG1 a cells were measured by CCK-8 method. LDH releasing assay was used to detect the cytotoxic effects of activated immune cells, NNAVC combined with activated immune cells on KG1 a cells and the sensitivity of KG1 a treated with NNAVC to activated immune cells. The results showed that the inhibitory rate of NNAVC on KGla cells increased with the concentration enhancement, the cytotoxicity of activated immune cells atthe different effector to target（E： T） ratios（6.25： 1,12.5： 1,25： 1） on KGla cells were 12.30%, 24.85% and 52. 26%. The cytotoxicity of NNAVC combined with activated immune cells at the different E： T cell ratios （ 10： 1, 20 ： 1 ） on KG1 a cells were 56. 21% and 85.59%, which were higher than that of NNAVC or activated immune cells alone. The cytotoxicity of activated immune cells at the E： T cell ratio of 10：1 on KGla cells treated with NNAVC at different concentrations were 25.65% ,31.33% ,28.63% and 16. 78% ,respectively, and that at the E： T cell ratio of 20：1 were 40. 62% ,44.70% ,44. 62% and 40. 72%. It is concluded that：both of NNAVC and activated immune cells have lethal effect on KG1 a cells, and the combination of NNAVC and activated immune cells can strengthen their effect on KG1 a.