摘要
基于8-17 DNA酶的催化切割特性和类辣根过氧化物酶DNA酶的氧化还原反应催化特性,发展了一种新型核酸酶催化放大传感体系,并用于Pb2+的比色检测。考察了K+浓度,pH值及反应时间对检测体系的影响。ABTS吸光度变化与Pb2+浓度在5.0~100 nmol/L范围内呈良好的线性关系,检出限为3.0 nmol/L。此外,因Pb2+酶的特异性,本方法对Pb2+具有良好的选择性。
Based on the catalytic cleavage of 8- 17 DNA enzyme (DNAzyme) and the catalytic redox of horseradish peroxidase (HRP)-mimicking DNAzyme, a novel DNAzyme catalytic amplification biosensing platform has been proposed for the colorimetric detection of lead ions ( Pb2+ ). The effects of K+ concentration, pH value and incubation time on the detection system were investigated. The system exhibited a dynamic response range for Pb2+ from 5 to 100 nmol/L with a detection limit of 3 nmol/L. In addition, the selectivity of the sensor for Pb2+ against other environmentally related metal ions was outstanding.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2013年第5期670-674,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(Nos.21075032,21005026,21135001)
关键词
DNA酶
比色检测
催化放大
生物传感
铅离子
Deoxyribonucleic acid enzyme
Colorimetric detection
Catalytic amplification
Biosensing
Leadion(II)