LC-MS法测定大鼠血浆中的冬凌草甲素含量

Quantitative Determination of Oridonin in Rat Plasma by LC-MS

建立大鼠血浆中冬凌草甲素的L C-M S测定方法。用C18柱(150mm×2.0mm,5Lm)色谱柱,乙腈-水(V/V=50∶50)为流动相,乙酸乙酯作为萃取剂,HPL C/电喷雾离子源质谱检测(HPL C/ESIM S)。该方法测定乙酸乙酯萃取的冬凌草甲素在0.018—9.2Lg·mL-1浓度范围内线性关系良好(R2=0.9992)。定量下限为0.018Lg·mL-1,提取回收率和RSD分别为100.56%—101.74%和8.88%。采用L C-M S法检测乙酸乙酯萃取的大鼠血浆中的冬凌草甲素,可为进一步深入研究冬凌草甲素在人体内药代动力学特征提供可靠检测手段。

A LC-MS method for quantitative determination of the oridonin in rat plasma was established. Acetonitrile-Water （V/V = 50 ： 50） flowphase system, C18 column （150mm×2.0mm,5μm）,HPLC/Electrospray ionization mass spectrometric detection （HPLC/ESI-MS） and acetic ether were explored as extractant. The assay was found to be linear for oridonin concentrations in the range 0. 018μg·mL^-1to 9.2μg·mL^-1,with a lower limit 0. 018μg·mL^-1 quantification. The extractive recovery and RSD were 100. 56%-101. 74% and 8. 88%. This method provided an effective and reliable LC-MS method to evaluate the pharmaeokinetics profile of oridonin in rat plasma. It may be a reliable technology to study pharmacokineties of oridonin in a human body.