光谱法研究维生素C和拉米夫定与血清白蛋白的相互作用

Interaction Between Vitamin C and Lamivudine in Presence of Bovine Serum Albumin by Spectrometry

应用光谱法研究在模拟动物生理条件(pH=7.40的缓冲溶液)下,维生素C(Vitamin c)和拉米夫定(Lamivudine)对牛血清白蛋白(BSA)的荧光猝灭作用。建立了利用荧光光谱法研究维生素C和拉米夫定间相互作用的方法。用Stern-Volmer方程处理实验数据,发现BSA与药物反应生成新的产物,以静态猝灭为主要猝灭机理,药物与蛋白结合位点数约为1,药物间存在相互作用,增大了药物与蛋白间的结合常数、稳定性,减少了游离型药物含量,造成药效减弱。

In the aqueous solution of pH=7.40 Tris-HCl,both vitamin C and lamivudine can quench fluorescence of bovine serum albumin（BSA）. According to this,studies were established on the interaction between vitamin C and lamivudine by fluorescence spectrum. It was proved that the interaction between the drugs increases the binding stability, of the drug and protein. At the same time, reduce of the free drug concentration will reduce the effect of the drugs. The research showed that the quenching mechanism of the combination of bovine serum albumin and drug is a static procedure.