摘要
建立高效液相色谱法测定罗氟司特的有关物质。采用Shimadzu VP-ODS C18色谱柱,甲醇-醋酸铵缓冲液(pH 4.5)(60∶40)为流动相,检测波长为245nm,柱温25℃。罗氟司特检出限为9.3×10-10g·mL-1,主成分与其相邻杂质峰分离良好。本方法简便、准确、灵敏度高、专属性好,可用于罗氟司特有关物质的测定。
A method was established to determine the content of related substance of roflumilast. A shimadzu VP-ODS Cls column was used with methanol-ammonium acetate buffer （pH= 4. 5） （60 ： 40） at column temperature of 25 C as the mobile phase. The detection wavelength was set at 245nm. The detection limit for roflumilast was 9.3 × 10^-10g · mL^-1 Good resolution for roflumilast and related, substances was obtained. The method is simple, accurate, reliable and sensitive for the quality control of roflumilast.
出处
《光谱实验室》
CAS
2013年第6期3122-3126,共5页
Chinese Journal of Spectroscopy Laboratory
