宫颈癌标本中HSV2和衣原体的检测被引量：1

Detection of HSV2 and Chlamydia in cervical cancer specimens

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摘要目的:确定人单纯疱疹病毒2(HSV2)和衣原体DNA在标本宫颈腺瘤中是否出现,以及与HPV感染之间的相关性。方法:从257份宫颈腺瘤石蜡包埋标本提取DNA,采用PCR法对DNA进行扩增,并进行HPV基因分型,检测CT和HSV-2 DNA的阳性率。结果:257份石蜡包埋标本中,67份标本能检出β-globin DNA序列,其中HPV DNA阳性率为79.4%,其中HPV18和HPV16分别为51%和34%。在所有这些标本中没有检测到HSV2和衣原体DNA。结论:没有检测到HSV2和衣原体DNA整合到宫颈腺癌的细胞基因组,也没有发现它们是HPV所致的宫颈腺癌的辅助致病因子。 Objective： To explore the appearance situation of Herpes simplex virus type 2 （HSV -2） and Chlamydia DNA in cervi- cal adcnoma specimens and their correlations with human papillomavirus （ HPV ） infection. Methods： DNA was abstracted from 257 paraffin -embedded cervical adenoma specimens, PCR was used to amplify DNA, HPV genetic typing was conducted, the positive rates of Chlamydia trachomatis （CT） and HSV -2 DNA were detected. Results： A total of 257 specimens were detected, and 13 -globin DNA se- quence was found in 67 specimens, the positive rate of HPV DNA was 79.4% , the positive rates of HPV18 and HPV16 were 51% and 34%, respectively. HSV2 and CT DNA were not found in all the specimens. Conclusion： In the study, HSV -2 DNA and CT DNA were not found to be integrated to the genome of ad ogy of adenocarcinoma of uterine cervix. of the uterine cervix and they do not seem to be a co - factor for HPV on the etiol-

作者杨君王慧玲华方方

机构地区新乡医学院第一附属医院妇产科

出处《中国妇幼保健》 CAS 北大核心 2013年第31期5197-5199,共3页 Maternal and Child Health Care of China

关键词宫颈癌单纯疱疹病毒2 衣原体 Cervical cancer Herpes simplex virus type 2 Chlamydia

分类号 R737.33 [医药卫生—肿瘤]

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