常温CANON反应器内微生物群落结构及生物多样性

Bacterial community structure and biodiversity of CANON reactor at room temperature

为研究基于亚硝化的全程自养脱氮(CANON)工艺中微生物群落结构及种属特征,从常温条件下稳定运行的CANON反应器中采集生物膜样品,并基于聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术,分别对总细菌、氨氧化细菌和厌氧氨氧化菌进行群落结构解析.结果表明:反应器中与亚硝化作用有关的功能菌为亚硝化单胞菌属(Nitrosomonas)和亚硝化螺菌属(Nitrosospira),与厌氧氨氧化作用有关的功能菌是Candidatus Brocadia属和CandidatusKuenenia属,未检测到亚硝酸盐氧化细菌,且系统内氨氧化细菌的生物多样性明显高于厌氧氨氧化菌.此外,反应器中还存在其他微生物,如Shewanella、Pseudomonas、Ignatzschineria、Dechloromonas以及属于Clostridia、α-Proteobacteria、Bacillales的uncultured bacteium等.

To investigate bacterial community structure and biodiversity of a CANON reactor that was operated stably at room temperature,total DNA was extracted from the biofilm for PCR-DGGE analysis.The results of microbial community analysis showed that the ammonia oxidizing bacteria Nitrosomonas and Nitrosospira,together with the anaerobic ammonia oxidizing bacteria Candidatus Brocadia and Candidatus Kuenenia were detected as the functional bacteria while nitrite-oxidizing bacteria（NOB） was not found in the CANON system,and the biodiversity of AOB was significantly higher than anammox bacteria.Besides,some other bacteria like Shewanella,Pseudomonas,Ignatzschineria,Dechloromonas and some uncultured bacterium related to Clostridia,α-Proteobacteria and Bacillales also existed in the reactor.