摘要
分离鉴定大鼠灌胃丹酚酸C后血浆和尿液中的代谢产物。代谢样品用强阴离子交换小柱固相萃取方法进行制备后,采用高效液相-四极杆-飞行时间串联质谱(HPLC-ESI/Q-TOF MS/MS),在负离子模式下进行检测,获取数据进行结构鉴定。从大鼠血浆和尿液中检测到丹酚酸C原形成分和5个代谢产物,分别鉴定为caffeic acid(M1),monomethyl-salvianolic acid C-monoglucuronide(M2),dimethyl-salvianolic acid C-monoglucuronide(M3),dimethyl-salvianolic acid Cmonoglucuronide(M4),dimethyl-salvianolic acid C(M5)。结果表明丹酚酸C在大鼠体内的主要代谢途径是甲基化和葡萄糖醛酸化反应。
The objective of this study was to separate and identify the metabolites in rat plasma and urine after oral administration of salvianolic acid C, a phenolic acid in Salvia miltiorrhiza（Danshen）. Solid-phase extraction method with strong anion exchange was used to separate the metabolites from the biological matrix. High-performance liquid chromatography coupled with electrospray quadrupole time-of-flight tandem mass spectrometry（HPLC-ESI/Q-TOF MS/MS）analysis was performed to identify the metabolites. Five metabolites were identified as caffeic acid（M1）, monomethyl-salvianolic acid C-monoglucuronide（M2）, dimethyl-salvianolic acid C-monoglucuronide（M3）, dimethyl-salvianolic acid C-monoglucuronide（M4）, dimethyl-salvianolic acid C（M5）, as well as the prototype of salvianolic acid C detected in plasma and urine. The results indicated that methylation and glucuronidation might be the two main metabolic pathways of salvianolic acid C.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2013年第5期442-446,共5页
Journal of China Pharmaceutical University
基金
国家科技支撑计划资助项目(No.2008BAI51B01)
国家自然科学基金资助项目(No.81073006)~~
