HPLC法测定不同产地和种属麻黄中麻黄碱与伪麻黄碱的含量

Determination of ephedrine and pseudoephedrine in Herba Ephedrae from different habitats and species by HPLC method

目的:建立麻黄药材中麻黄碱与伪麻黄碱的HPLC含量测定方法,并将其应用于不同产地和种属麻黄的测定。方法:采用Luna C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.2%磷酸溶液(含0.05%三乙胺,4∶96,v/v)为流动相,流速1.0 mL/min,柱温30℃,检测波长210 nm。结果:麻黄碱和伪麻黄碱分别在0.04-0.51μg、0.07-0.97μg范围内线性关系良好;平均回收率分别为98.7%和99.2%,RSD分别为2.1%和1.65%。结论:本方法结果准确,操作简单,数据可靠,可用于麻黄药材中麻黄碱与伪麻黄碱的含量测定,同时也为麻黄药材的鉴别和质量评价提供了依据。

Objective：To establish a HPLC method for determining ephedrine and pseudoephedrine in Herba Ephedrae from different habitats and species.Methods：The separation was achieved on a Luna C18 column（250 mm ×4.6 mm,5 μm） at 30 ℃ using acetonitrile-0.2% phosphate solution（containing 0.05% triethylamine）（4∶96,v/v） as the mobile phase at a flow rate of 1.0 mL/min,and 210 nm as the detection wavelength.Results：The linear range of ephedrine and pseudoephedrine was 0.04-0.51 μg and 0.07-0.97 μg,respectively.The average recovery for ephedrine and pseudoephedrine was 98.7% and 99.2% with RSD of 2.1% and 1.65%,respectively.Conclusion：The developed method is accurate,simple and applicable for the determination of ephedrine and pseudoephedrine in Herba Ephedrae,and also provides a basis for the identification and quality evaluation of this medicinal herb.