补肾活血通淋方对良性前列腺增生症大鼠模型Bax、Bcl-2及Bax/Bcl-2的影响

Effects of Bushen Huoxue Tonglin Decoction on the Bax,Bcl-2 and Bax/Bcl-2 in rat models with benign prostatic hyperplasia

目的:观察补肾活血通淋方对良性前列腺增生症大鼠模型Bax、Bcl-2及其比值的影响。方法:72只Wistar大鼠随机分为6组,即假手术组、模型组、保列治组、补肾活血通淋方低、中、高剂量组。除假手术组外,余组采用摘除大鼠双侧睾丸后连续注射丙酸睾丸酮30d的方法建立模型,在造模的同时给药组每天灌胃给药1次,连续30d。采用RT-PCR法测定Bax mRNA和Bcl-2mRNA的表达强度并计算其比值。结果:各治疗组Baxm RNA的相对表达量均高于模型组(P<0.05),保列治组、补肾活血通淋方中、高剂量组BaxmRNA的相对表达量均高于补肾活血通淋方低剂量组(P<0.05),补肾活血通淋方高剂量组Bax mRNA的相对表达量高于保列治组、补肾活血通淋方中剂量组(P<0.05),各治疗组Bcl-2 mRNA的相对表达量均低于模型组(P<0.05),补肾活血通淋方中、高剂量组BaxmRNA的相对表达量均低于补肾活血通淋方低剂量组和保列治组(P<0.05)。各治疗组Bax/Bcl-2均高于模型组(P<0.05),保列治组、补肾活血通淋方中、高剂量组Bax/Bcl-2均高于补肾活血通淋方低剂量组(P<0.05)。补肾活血通淋方高剂量组Bax/Bcl-2高于补肾活血通淋方中剂量组和保列治组(P<0.05)。结论:补肾活血通淋方可以上调大鼠良性前列腺增生症模型Bax的表达,下调Bcl-2的表达,并能提高Bax/Bcl-2的比值,这可能是其治疗前列腺增生症的机制之一。

Objective：To observe the effect of Bushen Huoxue Tonglin Formula（BSHXTLF） on gene expression of Bax and Bcl-2 and ratio of Bax and Bcl-2 in Benign Prostatic Hyperplasia（BPH） rats. Methods ：72 rats were randomly divided into six groups ： sham-operated group, model group, Proscar group, low dose group, middle dose group and high dose group. The rats model was established by injecting testosterone propionate 30 days after removing testis exclude sham-operated group. The drugs were administrated once a day at the same time. RT-PCR was used to measure the gene expression of Bax and Bcl-2,the calculating the ratio. Results： The expression of Bax mRNA in the treatment groups was increased compared with model group（P 〈0. 05）. The expression of Bax mRNA in Proscar group, middle and high dose BSHXTLF group was increased compared with low dose BSHXTLF group （ P 〈 0.05 ）. The expression of Bax mRNA in high dose BSHXTLF group was increased compared with Proscar group and middle dose BSHXTLF group （P 〈 0. 05）. The expression of Bcl-2 mRNA in the treatment groups was decreased compared with model group （P 〈 0.05）. The expression of Bcl-2 mRNA in middle and high dose BSHXTLF group was decreased compared with low dose BSHXTLF group and Proscar group（ P 〈 0. 05）. Ratio of Bax and Bcl-2 of each treatment groups was increased compared with model group （ P 〈 0.05 ）. Ratio of Bax and Bcl-2 of Proscar group, middle and high dose BSHXTLF group was increased compared with low dose BSHXTLF group（P 〈 0.05 ）. Ratio of Bax and Bcl-2 of high dose BSHXTLF group was increased compared with middle dose BSHXTLF group and Proscar group （ P 〈 0. 05 ）. Conclusion ： BSHXTLF may increase gene expression of Bax and decrease gene expression of Bcl-2 and increase Ratio of Bax and Bcl-2. These are one of the mechanisms of BSHXTLF in the treatment of BPH.