HPLC法测定脂降宁片中大黄素的含量

Determination of Emodin in Zhijiangning Tablet by HPLC

目的 建立脂降宁片中大黄素含量的高效液相色谱测定方法。方法 Agilent Extend C18色谱柱(250×4.6mm,5μm),以甲醇-0.1%磷酸溶液(80:20)为流动相,检测波长为290nm。结果大黄素进样量在0.005025μg^0.1005μg的范围内呈良好的线性关系(r=0.9999),平均回收率为99.2%。结论本方法简单,准确,重复性好,可用于该药品的质量控制。

Objective To establish a RP-HPLC method for the determination of emodin in Zhijiangning tablet. Methods Agilent Extend C18 column was used. The mobile phase was methanol-0.1% phosphoric acid（80：20）. The detection wavelength was set at 290nm. Results A good linearity was obtained in the range of 0.005025μg to 0.1005μg（r=0.9999）.The average recovery was 99.2%. Conclusion The method is simple,accurate and repeatable and can be used for the quality control.