星点设计-效应面法优选还阳参总酚酸的提取工艺

Optimization of Extraction Technology for Total Phenolic Acids from Crepis turczaniowii by Central Composite Design-Response Surface Methodology

目的:优选还阳参总酚酸的提取工艺。方法:在单因素试验基础上,采用星点设计-效应面法,选取乙醇体积分数、提取时间和溶剂倍量为自变量,还阳参总酚酸的提取量为因变量,对自变量各水平进行多元线性回归和二项式拟合,选择较佳工艺条件,并进行预测分析。采用紫外分光光度法测定总酚酸含量。结果:还阳参总酚酸的最佳提取工艺为加35倍量70%乙醇提取3次,每次50 min。预测值与理论值的偏差-2.28%,二项式拟合复相关系数0.939 6。结论:采用星点设计-效应面法优选的提取工艺稳定可行、精密度高、可预测性好,适用于还阳参总酚酸的提取。

Objective： To optimize extraction process of total phenolic acids from Crepis turczaniowii. Method： Based on single factor tests, central composite design-response surface methodology was adopted to optimize extraction process of total phenolic acids from C. turczaniowii, independent variables were ethanol concentration, reflux time and solvent folds, dependent variable was extraction rate of total phenolic acids. Linear or no-linear mathematic models were used to estimate relationship between independent and dependent variables,optimum extraction process was optimized, then prediction was carried out through comparing the observed and predicted values. The content of total phenolic acids was determined by UV. Result： Optimum extraction technology conditions were ： extracted 3 times with 35 times the amount of 70% ethanol for 55 minutes each time. Regression coefficient of binomial fitting complex model was as high as 0. 936 9, bias between the observed and predicted values was -2.28%. Conclusion： Optimum extraction process was reasonable, stable and feasible, it was suitable for development and utilization of total phenolic acids from C. turczaniowii.