正交试验优选黔产铁苋菜中没食子酸的提取工艺

Optimization of Extraction Process for Gallic Acid in Acalypha australis from Guizhou by Orthogonal Test

目的:优选黔产铁苋菜中没食子酸的提取工艺。方法:采用HPLC测定铁苋菜中没食子酸含量,Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.05%磷酸(4∶96),检测波长270 nm,柱温室温,流速1 mL·min-1,进样量10μL。以没食子酸转移率为指标,通过单因素试验筛选提取溶媒和提取方法,采用正交试验考察液固比、乙醇体积分数、提取温度、提取时间对提取工艺的影响。结果:选择乙醇为提取溶媒,采用回流法提取。最佳提取工艺条件为加30倍量70%乙醇于90℃提取2次,每次4 h,没食子酸平均转移率7.888 mg·g-1(RSD 1.60%)。结论:优选的提取工艺稳定可行,为黔产铁苋菜的开发利用提供实验依据。

Objective： To optimize extraction process of gallic acid in Acalypha australis from Guizhou Method： The content of gallic acid from A. australis was determined by HPLC, Diamonsil C1s column （4.6 mm x 250 mm, 5 μm）, mobile phase of acetonitrile-0.05% phosphoric acid （4： 96）, detection wavelength 270 rim,column temperature at room temperature, flow rate 1 mL ·min-1, injection volume 10 μL. With transfer rate of gallic acid as index, single factor tests were adopted to screen extraction methods and solvents, effects of ratio of solid-liquid, ethanol concentration, extraction temperature and time on extraction technology were investigated by orthogonal test. Result： The best extraction process of gallic acid was as following： extracted 2 times with 30 times the amount of 70% ethanol at 90 ℃, 4 hours per time. Average transfer rate of gallic acid was 7. 888 mg ·g ^-1 （RSD 1.60% ）. Conclusion： This optimized extraction process was stable and feasible, it could provide experimental basis for development and utilization of A. australis from Guizhou.