土木香趁鲜切制与传统切片方法的比较

Comparison of Traditional Slicing Method and Fresh-Cut Method for Inulae Radix

目的：探讨鲜切土木香饮片的可行性，对土木香传统切片工艺进行改良。方法：以土木香内酯、异土木香内酯及总黄酮含量为指标，通过正交试验考察含水量、干燥方式及切片厚度对土木香直接鲜切饮片工艺的影响，并与传统切片工艺进行比较。采用紫外分光光度法测定总黄酮含量；HPLC测定土木香内酯和异土木香内酯含量，色谱条件为SHIMADZUC18色谱柱（4．6mm×200mm，5μm），流动相乙腈-0．1％磷酸溶液（50：50），柱温30℃，流速1．0mL·min^-1，检测波长220nm／1，进样量20μL。结果：优选的鲜切饮片工艺为土木香药材在含水量为35％时切片，饮片厚度2．5～3．0mm，阴干。鲜切饮片中土木香内酯、异土木香内酯及总黄酮质量分数分别为（22．41±1．38），（12．87±0．73），（15．04±0．64）mg·g^-1，传统工艺切制饮片中则依次为（22．94±1．24），（13．24±0．83），（14．12±0．58）mg·g^-1。结论：趁鲜切制土木香饮片产品质量稳定，具有推广意义。

Objective： To evaluate feasibility of fresh-cut process for Inulae Radix and improve traditional slicing process of it. Method： The contents of alantolactone, isoalantolactone and total flavonoids were employed as indicators, effects of water content, drying styles and slice thickness on fresh-cut process of Inulae Radix were investigated by orthogonal test, then compared with conventional slicing process. The content of total flavonoids was determined by UV; The contents of alantolactone and isoalantolactone were determined by HPLC, chromatographic conditions were as follows： SHIMADZU C18 column （4.6 mm x200 ram, 5 μm） , mobile phase of acetonitrile-0. 1% phosphoric acid （50： 50）, column temperature 30 ℃ , flow rate 1.0 mL ·min ^-1, detection wavelength 220 nm, injection volume 20 μL. Result： Optimized fresh-cut process was： water content 35% , drying styles of shade drying, slice thickness 2.5-3.0 mm. The contents of alantolactone, isoalantolactone and totalflavonoids from fresh-cut pieces were （22.41±1.38）, （12.87±0.73）, （15.04±0.64） mg·g^-1, but in traditional slicing process were （22.94±1.24）, （13.24 ±0.83）, （14.12 ±0.58） mg·g^-1 Conclusion： Fresh-cut process of Inulae Radix was stable and meaningful.