高压炮制对何首乌中有效成分含量的影响

Influence of High Pressure Processing on Contents of Active Ingredients from Polygoni Multiflori Radix

目的:考察高压炮制对何首乌中大黄素、大黄素甲醚及二苯乙烯苷含量的影响。方法:何首乌采用高压清蒸法炮制,考察不同蒸制时间对何首乌中蒽醌类成分、二苯乙烯苷含量的影响,并观察其在炮制过程中性状变化。采用HPLC测定大黄素、大黄素甲醚含量,色谱条件为Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1%磷酸(80∶20),流速1.0 mL·min-1,检测波长254 nm,柱温30℃,进样量10μL;二苯乙烯苷的HPLC测定条件为Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流速1.0 mL·min-1,流动相乙腈-水梯度洗脱,检测波长320 nm,柱温30℃,进样量10μL。结果:高压蒸制0,1,2,3,4,5,6 h时二苯乙烯苷质量分数(n=2)依次为4.55%,3.54%,2.20%,1.87%,1.52%,1.15%,0.76%。随着蒸制时间的增加,何首乌中结合蒽醌、二苯乙烯苷含量逐渐下降,游离蒽醌含量在一定蒸制时间内随蒸制时间的增加而增多。结论:何首乌作为补益药应用时宜采用高压蒸制4 h炮制品。

Objective： To investigate effects of high pressure processing on contents of emodin, physcion and 2, 3, 5, 4＇-stilbene glucoside from Polygoni Multiflori Radix. Method： Polygoni Multiflori Radix was processed by high pressure steaming method, effects of different steaming time on contents of anthraquinones and 2, 3, 5, 4＇-stilbene glucoside were investigated, and properties change during processing was observed. The contents of emodin and physeion were determined by HPLC, chromatographic conditions were as follows： Diamonsil Cls column （4.6 mm x250 ram, 5μm） , mobile phase of methanol-0.1% phosphoric acid （80： 20） , flow rate 1.0 mL -min-I, detection wavelength 254 nm, column temperature 30 ℃ , injection volume 10 μL. HPLC conditions of 2, 3, 5, 4＇-stilbene glucoside were as follows： mobile phase of acetonitrile-water gradient elution, detection wavelength 320 nm; other conditions were the same as these above conditions. Result： The content of 2, 3, 5, 4＇-stilbeneglucosidewere4.55%, 3.54%, 2.20%, 1.87%, 1.52%, 1. 15%, 0.76% after high pressure steamed 0, 1, 2, 3, 4, 5, 6 h. By increasing of steaming time, contents of incorporating anthraquinones and 2, 3, 5, 4＇-stilbene glucoside from Polygoni Multiflori Radix gradually decreased, but the content of free anthraquinones increased. Conclusion： As tonics applications, Polygoni Multiflori Radix should be heated for four hours under high pressure.