云南野生抚育粗茎秦艽药材的品质评价

Quality Evaluation of Wildlife Tending Gentiana crassicaulis in Yunnan Province

目的:研究野生抚育粗茎秦艽药材的有效成分和化学指纹图谱,以期评价其质量,为粗茎秦艽药材的GAP种植提供科学依据。方法:采用Zorbax SB C18(4.6 mm×250 mm,5μm)色谱柱,流动相甲醇-水,梯度洗脱,流速1 mL·min-1,检测波长238 nm,柱温为25℃。利用国家药典委员会出版的《中药色谱指纹图谱相似度评价系统》2004 A版软件,对15批3年生野生抚育粗茎秦艽药材进行指纹图谱分析。结果:15批粗茎秦艽药材环烯醚萜苷类有效成分及浸出物含量符合2010年版《中华人民共和国药典》要求,各样地药材化学指纹色谱相似度>0.981,质量均一性好。结论:方法具有良好的精密度、重复性、稳定性,可用于粗茎秦艽药材的质量综合评价。野生抚育模式药材可作为粗茎秦艽主要种植发展模式,对云南建设"云药之乡"和发展道地药材具有重要的现实意义。

Objective： Radix Gentiana crassicaulis is the major cultivated Gentiana species in Yunnan Province. Wildlife tending is a major cropping pattern. In this study, an improved and comprehensive HPLC fingerprints were developed for the quality evaluation of G. crassicaulis, together with the simultaneous determination of four iridoid and secoiridoid glycosides. Loganic acid, swertiamarin, gentiopicroside, sweroside were also detected. Method： HPLC separation was performed on an Agilent Zorbax SB Cls column. The mobile phase was methanol and water with linear gradient change of methanol from 0 to 40% in 20 min. The flow rate was 1.0 mL ·min^ -1 , and the detection wavelength was set at 238 rim. Result： The HPLC chromatograms of fifteen samples from different regions of China showed a similar pattern. Thirteen peaks were selected as the characteristic peaks to evaluate the similarities among different G. crassicaulis samples. Conclusion： This is the first report on an HPLC method for the simultaneous determination of four bioactive components and fingerprint analysis of wildlife tending G. crassicaulis. The results indicate that this comprehensive method is suitable for evaluate the quality of G. crassicaulis for GAP planting.