黄杞叶化学成分的定量分析

Quantitative Analysis of Chemical Compositions in Leaves of Engelhardtia roxburghiana

目的:测定黄杞叶不同提取物中主要化学成分的含量。方法:分别以芦丁、没食子酸和齐墩果酸为对照品,采用分光光度法测定黄杞叶95%乙醇提取物及水提物中总黄酮、总多酚以及总皂苷的含量。结果:黄杞叶95%乙醇提取物中总黄酮含量为48.01%、总多酚含量为34.01%、总皂苷含量为20.91%;黄杞叶水提物中总黄酮含量为42.04%、总多酚含量为33.25%、总皂苷含量为17.84%。结论:方法简便、准确、稳定性好、重复性好,可用于黄杞中三大类化学成分的含量测定。

Objective： To determine the quality of chemical compositions in the different extracts of Engelhardtia roxburghlana leaves. Method： Rutin, gallic acid and oleanolic acid were used as the control samples. Speetrophotometry was used to determine the content of total flavonoids, total polyphenol and total saponins in the 95% alcohol extract and water extract of the leaves of E. roxburghiana. Result： The 95% alcohol extract content of total flavonoids, total polyphenols and total saponins were 48.01% , 34.01% and 20.09% respectively. The content of water extract was 42.04% , 33.25% and 17.84% respectively. Conclusion： This method is simple, accurate and reliable, which could be used to determine the quality of the three major chemical compositions in E. roxburghiana.