黑逍遥散对Aβ25-35诱导AD大鼠模型脑组织和血清SOD，GSH-Px及MDA的影响

Effects of Hei Xiaoyao San on SOD,GSH-Px and MDA of Brain Tissue and Serum in Alzheimer's Disease Rat Model Induced by Aβ_(25-35) Fragments

目的:观察黑逍遥散对β淀粉样蛋白(Aβ25-35)诱导阿尔芡海默病(AD)模型大鼠脑组织和血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)的影响,探讨黑逍遥散防治AD的作用及其机制。方法:雄性SPF级SD大鼠110只,先按随机数字表挑选16只大鼠作为假手术组,其余大鼠手术7 d后进行水迷宫测试,将测试合格的大鼠再按照随机数字表法分为5组:模型组、阳性对照组、黑逍遥散高、中、低剂量组,每组14只。假手术组14只。采用Aβ25-35海马区注射诱发大鼠脑内神经元损伤,复制大鼠AD模型;造模后7 d,黑逍遥散各组大鼠分别按低、中、高剂量(4.2,8.50,17.0 g·kg-1·d-1)ig给药,阳性对照组给予石杉碱甲片溶液0.02 mg·kg-1·d ig,模型组、假手术组ig给予等容量双蒸水,每日上午1次,连续治疗28 d。末次给药后处死大鼠并采集血清和脑标本,比色法测大鼠血清和脑匀浆SOD,MDA,GSH-Px活性;结果:与假手术组比较,模型组大鼠血清和脑组织匀浆中的SOD,GSH-Px显著降低,MDA含量显著增高;与模型组比较,黑逍遥散能显著升高Aβ25-35诱导AD模型大鼠血清和脑组织中SOD与GSH-Px活性、降低MDA含量(P<0.05)。结论:黑逍遥散通过抗氧化作用起到保护AD模型大鼠的作用。

Objective： To explore the influence of Hei Xiaoyao San on superoxide dismutase （SOD） , gluthathione peroxidase （GSH-Px） and malondialdehyde （MDA） of brain tissue and serum in Alzheimer＇s disease rat model induced by fl-amyloid protein （Aβ25-35） fragments so as to discuss the role and mechanisms involved in Hei Xiaoyao San in the prevention and treatment of Alzheimer＇s disease （AD）. Method： The SD rats （n = 94） were randomly divided into sham surgery rats （n = 16） group and model group （n =94） , and all rats of model group received water maze tests for 7 days. These eligible rats were randomly divided 5 groups, model group,positive control group, Hei Xiaoyao San high, medium and low dose groups, 14 rats in each group. Aβ25-35 fragments was injected into the hippocampus to damage brain neuron to make the rat＇s AD model. 7 days later, all rats of Hei Xiaoyao San group were orally administered Hei Xiaoyao San at low, medium and high doses （4.2, 8.50, 17.0 g-kg 1-d-1）, the positive control group was orally given huperzine A solution 0.02 mg ·kg-l .d, model group and sham group received an equal volume double distilled water daily for 28 d. After the last administration, the rats were sacrificed and serum and brain samples collected, and the serum and brain homogenate stration of SOD, MDA, GSH-Px were examined by means of chemistry chromatometry. Result： Compared with the sham group, serum and brain tissue homogenate SOD, GSH-Px of model group decreased significantly, MDA content increased significantly （P 〈 0.05）. Compared with the model group, Hei Xiaoyao San obviously increased the activities of SOD and GSH-Px and reduced the content of MDA （P 〈 0.05） in serum and brain tissue. Conclusion： Hei Xiaoyao San can protect rat＇s AD model from damage, and the mechanisms of which may be related to anti-free radicals.