藏药松生等醇提物不同极性提取部分体外抗氧化活性研究

Antioxidant Effect of Each Part of Ethanol Extrac of Rhamnella gilgitica in vitro

目的:通过体外还原力和抗氧化评价方法研究藏药松生等提取物的抗氧化活性。方法:将松生等用70%乙醇浸泡、提取、浓缩所得浸膏依次用石油醚,二氯甲烷,乙酸乙酯,正丁醇、乙醇进行萃取,各组分都配制成适当稀释倍数的溶液,通过普鲁士蓝法、Fenton法和,1,1-二苯基-2-三硝基苯肼(DPPH)法分别测定各组分的还原力,·OH自由基的清除能力以及抗氧化的活性,并计算各组分的IC50。结果:3种抗氧化实验表明乙酸乙酯、正丁醇、乙醇部分都具有明显抗氧化的作用。其中用DPPH法测得的IC50分别是乙酸乙酯0.060 5 g·L-1,正丁醇0.076 3 g·L-1,乙醇0.083 3 g·L-1。结论:提取物清除率与样品浓度存在明显的量效关系,浓度大于1.0 g·L-1时其还原能力几乎接近于维生素(VC),清除能力接近甚至超过60%。其中在大极性溶液层还原力和抗氧化的能力最强。

Objective： To study the scavenging activity in vitro of each part of ethanol extract of Rhamnella gilgitica Mansfeild et Melch. Method： R. gilgitica was extracted with 70% ethanol, then was extracted by petroleum ether, dichloromethane, ethyl acetate, n-butyl alcohol, ethanol the each part was configured to the appropriate solution, Prussian blue method Fenton method and 2, 2-diphenyl-l-picrylhydrozyl （DPPH） were used to measure the antioxidantactivity. Result： The ethyl acetat of R. gilgitica showed obvious scavenging effects, ICso of the ethyl acetate, n-butyl alcohol and ethanol extract of R. gilgitica with De, n-butyl alcohol and ethanol extractPPH was 0. 060 5, 0. 076 3, 0. 083 3 g·L^-1 , respectively. Conclusion： The scavenging activity showed a dose-response relationship, even reach 60% by the above 1.0 g·L^-1concentrations. The high polarity of solvents layer extract has the strongest free radical scavenging activity.