大黄水提物对成年大鼠子宫及卵巢的影响

Effect of Rhubarb Aqueous Extract on Uterus and Ovary Tissue in Adult Rats

目的：观察长期灌胃大黄对雌性大鼠子宫及卵巢的影响。方法：8周龄180～220g雌性Wistar大鼠90只，随机分为正常对照组、大黄水提取物1．0，2．0g·kg^-1组、各剂量组再分为灌胃30，40，50，60d组，每组10只，计算大鼠子宫、卵巢指数，观察子宫和卵巢的组织形态学变化。结果：大黄水提取物1．0g·kg^-1给药60d组子宫和卵巢指数分别为（0．21±0．058），（0．07±0．001），均显著低于正常对照组（0．26±0．038），（0．07±0．007）（P〈0．05）；大黄水提物2．0g·kg^-1。给药40，50，60d组子宫指数分别为（0．20±0．045），（0．19±0．054），（0．19±0．041），显著低于正常对照组（0．26±0．038）（P〈0．05或P〈0．01），大黄水提物2．0g·kg^-1给药30，40，50，60d组卵巢指数分别为（0．068±0．008），（0．065±0．007），（0．065±0．005），（0．061±0．006），均显著低于正常对照组（0．07±0．007）（P〈0．05或P〈0．01）；组织形态学显示，2．0g·kg^-150，60d组大鼠子宫内膜水肿，腺体减少；卵巢萎缩，卵泡数减少，卵泡内细胞排列不规则。结论：长期应用大黄可影响成年雌性大鼠的生殖功能，并有明显的剂量／时间依赖关系。

Objective ： To observe the long-term effect of Rhubarb on the uterus and ovary in female rats. Method： Ninety 8-week-old female rats （180-220 g） were randomly divided into nine groups： normal control group, and investigation groups （n = 10 each, administration of Rhubarb 1.0, 2.0 g·kg^-1 for 30, 40, 50, 60 d respectively）. Then the index of uterus and ovary were calculated, and the morphological changes in uterus and ovary were observed. Result： The indexes of uterus and ovary in Rhubarb 1.0 g·kg^-1 60 d group were lower than that in the control group obviously （P 〈0.05） ; the indexes of uterus in 2.0 g·kg^-1 groups （40, 50, 60 d） were lower than that in the control group obviously （P 〈0.05 or P 〈0.01 ）. While, the indexes of ovary in 2.0 g ·kg^-1 groups （30, 40, 50, 60 d） were lower than that in the control group obviously （P 〈0.05 or P 〈0.01）. The pathological examination showed that endometrial edema and glandular reduction, ovary atrophy, follicles reduction, and follicle cells irregular arrangment in 2.0 g ·kg^-1 groups （50, 60 d）. Conclusion： The long-term use of Rhubarb shows a certain degree of reproductive toxicity in female rats, and its impact is clear dose/time dependent.