乌梅丸及其拆方的镇痛作用

Analgesic Effect of Wumei Pill and its Disassembled Prescriptions

目的:观察乌梅丸及其拆方的镇痛作用。方法:实验动物分为对照组、模型组、全方组、寒热并用组、温热组、寒凉组、补益组、收敛组,各组给药依照拆方原则分别给予乌梅丸方13.3 g·kg-1,寒热并用方9.0 g·kg-1,温热方5.33 g·kg-1,苦寒方3.67 g·kg-1,收敛方2.67 g·kg-1,补益方1.67 g·kg-1水煎剂灌胃,空白组、模型组均给予生理盐水10 mL·kg-1。采用热板法和扭体法,观察乌梅丸及其拆方对小鼠的镇痛作用,三硝基苯磺酸/乙醇复制溃疡性结肠炎动物模型,酶联免疫法检测结肠黏膜前列腺素E2(PGE2)含量。结果:与对照组比较,乌梅丸全方组、寒热并用组和温热组药物能有效延长小鼠痛阈,降低小鼠扭体次数,降低溃疡性结肠炎大鼠结肠组织PGE2含量(P<0.05),且3组间无差异;补益组药物也表现出部分抑制疼痛的作用,但效果要弱于全方组(P<0.05)。结论:乌梅丸方有明显的镇痛作用,温热组药物为其镇痛的主要药物,补益组药物有部分镇痛作用。

Objective： To observe the analgesic effect of Wumei pill and its disassembled prescriptions on ulcerative colitis （UC） in rats. Method： Experimental animals were divided into control group, model group, Wumei pill group, cold and heat group, heat group, cold group, tonic group, convergence group. According to the decomposition principle, Each group was respectively given water decoction of Wumei pill （13.3 g·kg^-1）, cold and heat prescription （9.0 g ·kg^-1）, heat prescription （5.33 g·kg^-1）, cold prescription （3.67 g·kg^-1）, convergence prescription （2.67 g·kg^-1）, and tonic prescription （1.67 g ·kg^-1） by gavage. The control group and model group were given with saline （ 10 mL·kg^-1 ）. Analgesic effect of Wumei pill and its prescriptions were observed by hot plate and writhing. Ulcerative colitis model was established with trinitro-benzene-sulfonie acid （TNBS） /ethanol. The PGE2 content of colonic mucosa was determined by enzyme-linked immunosorbent assay. Result： Compared with blank group, the Wumei pill group, cold and heat group and heat group could prolong the pain threshold of mice, reduce the writhing times and decrease the PGE2 content of colonic mucosa in rats （P 〈 0.05）. Tonic group had partly analgesic effect but the effect was weaker than Wumei pill group （P 〈 0.05）. Conclusion： Wumei pill has obvious analgesic effect, while tonic group has partly analgesic effect. The drug of heat group play an very important role for analgesic effect.