柞蚕雄蛾油对运动训练大鼠睾酮含量、物质代谢及抗运动疲劳能力的影响

Effect of Tussah Male Moths Oil on Testosterone Content,Substance Metabolism and Exercise Capacity in Rats Receiving Exercise Training

目的:研究柞蚕雄蛾油对运动训练大鼠睾酮含量、物质代谢及抗运动疲劳能力的影响。方法:以大强度耐力训练大鼠为模型,55只42 d龄雄性Wistar大鼠为对象,随机分为5组:静止组(C组)、运动对照组(T组)、运动+低剂量柞蚕雄蛾油组(TML组),运动+中剂量柞蚕雄蛾油组(TMM组),运动+高剂量柞蚕雄蛾油组,每组10只(剔除不符合实验要求的大鼠)。每天ig 1次,柞蚕雄蛾油组剂量分为0.5,1,3 g·kg-1,ig体积为5 mL·kg-1,静止组、运动对照组ig等量生理盐水。42 d力竭游泳训练后,测定体重、力竭游泳时间及血睾酮及生化指标。结果:柞蚕雄蛾油各剂量组体重大于运动对照组(P<0.05);力竭游泳时间长于运动对照组(P<0.01);血清睾酮高于运动对照组(P<0.01);血清皮质酮水平各组间均无显著差异;血清睾酮与皮质酮比值变化与睾酮变化较为一致;肝糖原、肌糖原(P<0.05或P<0.01)高于运动对照组;促卵泡激素无明显影响;血清尿素氮低于运动对照组(P<0.01);血红蛋白高于运动对照组(P<0.01)。结论:柞蚕雄蛾油可以减轻血睾酮受高强度运动量的影响,并维持在正常生理水平;可以促进蛋白质合成,抑制氨基酸和蛋白质分解,提高运动训练大鼠血红蛋白含量和糖原的储备。

Objective： To study the effects of Tussah male moths oil on the content of testosterone, substance metabolism and anti-fatigue ability of rats. Method： By using the model of high-intensity endurance training, Wistar rats were divided into 5 groups, 10 in each group control group （C group） , motion control group （T group）, exercise ＋ ig low-dose Tussah male moths oil group （TML group）, exercise ＋ ig middle-dose Tussah male moths oil group （TMM group） , and exercise ＋ ig high-dose Tussah male moths oil group （TMH group）. Administration was performed once a day. The rats in Tussah male moths oil groups were gavaged with 0.5, 1, 3 g·kg^-1 with ig volume of 5 mL ·kg^-1. The rats in C and T groups were given same volume saline. After 42 days of exhaustive swimming training, body weight, swimming time and serum testosterone and other biochemical markers were measured. Result： The body weight of the rats in Tussah male moths oil groups was increased compared with T group （P 〈 0.05）, and the swimming time was longer than T group （P 〈 0.01 ）. Serum corticosterone was lower than the T group （P 〈 0.01 ）. Changes in the ratio of serum testosterone/cortieosteron were more consistent with testosterone changes among the groups. Liver glycogen （P 〈 0.05） , muscle glycogen （P 〈0.01 ） and hemoglobin （P 〈 0.01 ） in Tussah male moths oil group were higher than in T group, but the serum urea nitrogen was lower than the T group （P 〈 0.01 ）. Conclusion ： Tussah male moths oil can alleviate the impact of high-intensity exercise on serum testosterone, and maintaine it at normal physiological levels; it can also promote protein synthesis, inhibit degradation of amino acid and protein, and increase hemoglobin and glycogen reserves in rats exercise training.