摘要
研究超声波法提取石蒜中石蒜碱及加兰他敏的工艺条件,采用高效液相色谱(HPLC)同时检测两种物质。以石蒜碱和加兰他敏得率为指标,通过单因素试验分别考察浸提时间、浸提温度、超声作用时间和料液比对石蒜碱及加兰他敏得率的影响;并用正交试验得出最佳工艺。结果表明:由方差分析得到各因素的显著性依次是浸提时间>超声波作用时间>浸提温度;正交试验得出的最佳工艺条件为料液比1:10(g/mL),在70℃条件下浸提2.5h,然后在超波功率500W条件下提取30min,提取2次,石蒜碱和加兰他敏得率最高,分别达0.1561%、0.0253%。
Optimal conditions for the ultrasonic extraction of lycorine and galanthamine from red spider lily bulb were established in this study. The compounds were determined simultaneously by HPLC. The effects of extraction time, temperature, ultrasonic treatment time and material-to-solvent ratio on extraction efficiency were investigated. Analysis of variance showed that three extraction parameters in the decreasing order of importance were extraction time, ultrasonic radiation time and temperature. An extraction temperature of 70 ℃ and 2.5 h extraction followed by 30 min ultrasonic treatment repeated in sequence twice proved optimal, which provided maximum yields of lycorine and galanthamine, 0.1561% and 0.0253%, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第20期77-81,共5页
Food Science
基金
吉首大学研究生科研创新项目(JGY201227)
吉首大学校级科研课题(Jdy12043)
湖南省科技厅科技计划项目(2012TP4002-3)
湖南省高校"林产资源化学与林化产品开发"科技创新团队支持计划资助项目(湘教通(2010)212号)
关键词
石蒜
石蒜碱
加兰他敏
超声处理
red spider lily
lycorine
galanthamine
ultrasonic treatment
