黄芪超微粉HPLC指纹图谱的建立

HPLC Fingerprint Analysis of Ultra-Micro Powder of Astragali Radix

目的:建立黄芪超微粉高效液相色谱(HPLC)的指纹图谱,为其质量控制和科学评价提供依据。方法:采用Inertsil ODS-SP(4.6mm×250mm,5μm)色谱柱,甲醇-乙腈(1:1,V/V)为流动相A,超纯水为流动相B,进行梯度洗脱,流速0.8mL/min,柱温25℃,检测波长260nm。采用中药色谱指纹图谱相似度评价系统2004A进行相似度分析,并进行聚类分析。结果:建立了黄芪超微粉的指纹图谱,确定12个共有峰。聚类分析表明10批样品存在明显的南北地域差异。结论:此方法简便、准确、重复性好,可用于黄芪超微粉质量控制与评价。

Objective： To establish an high performance liquid chromatography fingerprint for quality control and evaluation of ultra-micro powder of Astragali Radix. Methods： Chromatographic separation was achieved on an Inertsil ODS-SP column （4.6 mm × 250 mm, 5 μm） with mobile phase A （methanol-acetonitrile, 1：1） and mobile phase B （ultra-pure water） at a flow rate of 0.8 mL/min. In addition, the detection wavelength and column temperature were set at 260 nm and 25 ℃, respectively. Results： Twelve peaks common to the established fingerprints were identified. Cluster analysis suggested apparent geographic differences between the samples from the north and the south. Conclusion： The proposed method is simple, accurate, repeatable and suitable for the quality control and evaluation of Astragali Radix ultra-micro powder.