茎直黄芪中毒中药复方预防解毒散急性和亚慢性毒性试验

Studies on the acute and sub-chronic toxicity of Chinese herbal compound antidote against locoweedpoisoning

为评价茎直黄芪中药复方解毒散的安全性,进行了茎直黄芪中药复方解毒散对小鼠的急性毒性和亚慢性毒性试验研究。采用改良Karber法测得茎直黄芪中药复方解毒散对小鼠的口服急性毒性LD50为(176.91±22.70)g/kg,95%可信限为156.60-202.00 g/kg,属低毒;采用经口灌胃法对小鼠连续给药7 d,7 d后对小鼠各项生理指标的测定结果表明,茎直黄芪中药复方解毒散在使用剂量范围内对小鼠的行为、体质量、饮食状况、生理和生化指标均无不良影响。在亚慢性毒性试验中,给药7 d后各给药组小鼠肾脏脏器系数与对照组差异不显著(P>0.05);各种酶指标与对照组亦无显著性差异(P>0.05)。由此可知,茎直黄芪中药复方预防解毒散毒性极低,使用安全。

Acute and sub-chronic toxicity of Chinese herbal compound antidote against locowee-dpoisoning was determined to evaluate its drug safety.In the acute toxicity test,different concentration of the compound was given to mice in 9 groups by intragastric administration for continuous 7 days,and the other 10 mice were given with normal saline as control.In the sub-chronic toxicity test,mice in 3 groups were administrated with high,middle and low dose of the compound by gavage for continuous 14 days.The results showed that the median lethal dose（LD50） of the herbal compound was（176.91±22.70） g/kg by Karber method,and it had no effect on the performance,behavior,blood index,biochemistry index and organ coefficients of mice after administration for 7 days.Sub-chronic toxicity test showed there was no significant difference in mice body weight,blood routine index,biochemistry index and morphological observation between experimental and control group on 1st and 14th after administration.It is concluded that Chinese herbal compound antidote is not toxic and has no potential adverse effect on mice for long-time use.