植物土传病害拮抗真菌的抗药性筛选

Resistance of UV induced mutants of antagonistic fungi to fungicides

从土壤中分离得到的 4种拮抗真菌( Trichoderma koningii、 T.pseudokonin-gii、T.harzianum和Gliocladium spp.)分别用紫外光诱变,得到抗氧氯化铜、扑海因和多菌灵的变异菌株。这些变异菌株对西瓜枯萎病菌(Fusarium oxysporum f.sp.melonis)和生菜菌核病菌(Sclerotinia minor)仍有较强的拮抗性。

When four antagonistic fungi (Trichoderma koningii, T. pseudokoningii, T. harzianum and Gliocladium spp. ) were irradiated with ultraviolet light for 30 min, mutants of strains were obtained. Mutants did not change the morphological characters and antagonistic activity to Fuserium axysporum f. sp. melonis and Sclerotinia minor when compared with the wild type. Resistance to iprodione and copper oxychloride was found to occur in all mutants. Some of them, such as mutants of T. Koningii, T. harzianum and Gliocladium spp, resisted to carbendazim. None of the mutants resisted to propiconazole.