抗生素对松材线虫病的影响

Effects of Antibiotics on the Pine Wilt Disease

利用某些抗生素的内吸性 ,筛选出可使黑松幼苗组织抑制B61 9菌的抗生素溶液浓度 ;进行了抗生素对松材线虫病影响的试验 ,在非无菌条件下 ,用松材线虫对幼苗接种 ,结果表明用抗生素溶液培养的幼苗未发生萎蔫 ,而用无菌水培养的幼苗均出现萎蔫病状 ;同时试验后检查抗生素溶液培养的幼苗 ,发现其组织内均有活线虫存在。这一结果不仅进一步说明了松材线虫病是一种由松材线虫和细菌共同侵染引起的复合侵染病害 ,而且首次用试验证明了通过控制致病细菌的方法可以控制松材线虫病。该结果为开发防治松材线虫病及早期诊断的新方法、培育抗松材线虫病的转基因松树新品种。

The results indicated that pine wilt disease was a disease complex involving the nematodes and bacteria. It was reported in this paper that the dosage of certain antibiotics in which growth of the B619 bacterium was inhibited in the tissue of the testing seedlings of P.thunbergii which was screened by using the systematic property of the antibiotics. Then under non aseptic conditions, inoculation of pine wood nematode on the testing seedlings was made. Results showed that no wilting symptom appeared on the seedlings cultured with antibiotics' solution, but all the seedlings cultured with aseptic water were wilted; After the test all the seedlings cultured with antibiotics solution were checked. And living nematodes existed in tissues of all checked seedlings. The results not only further demonstrated that the disease was a disease complex involving the nematodes and bacteria, but also showed that the pine wilt disease could be controlled by inhibiting the pathogenic bacteria for the first time. The results of this paper formed bases to develop new ways in controlling the disease and diagnosing the disease in the early phase, to culture new transgenic pines with anti pine wilt disease genes and to further study the mechanisms of the pine wilt disease.