摘要
从水稻田间采集自然感染白僵菌的水稻二化螟僵虫,分离纯化菌株,进行不同菌株生物学特性指标比较以及在室内对水稻二化螟3龄幼虫进行毒力测定,从中筛选出生长性状优良、对水稻二化螟毒力较强的菌株HN1、YJ2。其产孢量分别为19.03×107个/cm2、17.30×107个/cm2;14 h萌发率分别为93.67%、91.08%;营养生长量分别为2.822 mm/d、2.748 mm/d;对水稻二化螟3龄幼虫的校正死亡率分别为97.33%、96.00%。HN1、YJ2菌株各项生物学特性指标均显著高于实验室原有保藏菌株(CK),具有很好的生防潜力。
Nine strains ofBeauveria bassiana separated from Chilo suppressalis Walker were studied by comparing in sporulation, vegetative growth, spore germination rate and pathogenicity for 3rd instars larvae of the rice stem borer the Chilo suppressalis. The results showed that the strains HN1, YJ2 were recognized as the better strains for its high sporogenous ability (19.03 ×10^7 spores/cm2, 17.30 ×10^7 spores/cm2) on PDA medium, high germination rate (93.67%, 91.08%) in 14h, fast vegetative growth (2.822mm/d, 2.748 mm/d) and high mortality rate (97.33%, 96.00%), which were significantly higher than those of the CK strains. HN1, Y J2 strains could potentially be used in bio-control.
出处
《吉林农业科学》
CSCD
北大核心
2013年第5期44-47,共4页
Journal of Jilin Agricultural Sciences
基金
吉林省科技发展计划项目(20110216)
农业部公益性行业(农业)科研专项经费项目(201103002)
关键词
白僵菌
菌株筛选
水稻二化螟
Beauveria bassiana
Strain selection
Chilo suppressalis