针刺及川芎嗪对急性药物性聋豚鼠耳蜗内氧自由基生成影响实验研究

Effects of Tetramethylpyrazine and Acupuncture on Cochlear Free Radical Expression of Piunea Pigs with Acute Drug-induced Deafness

目的:探讨川芎嗪(TMP)、针刺单独应用及联合使用对庆大霉素(GM)致聋豚鼠耳蜗内氧自由基生成的影响。方法:50只健康豚鼠随机分为正常组、模型组、川芎嗪组、针刺组及TMP+针刺组,每组10只,正常组按2.5mL(/kg·d)剂量腹腔注射生理盐水,其余各组腹腔注射庆大霉素100mg(/kg·d),连续14天,TMP组、针刺组、TMP+针刺组同时灌服TMP和(或)针刺相关穴位。造模结束后迅速断头处死,取耳蜗标本。正常组与模型组动物于用药前及停药后第1天分别测试听觉脑干诱发电位(ABR)以判断造模是否成功;硫代巴比妥法检测血清丙二醛(MDA),黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)。结果:各治疗组与模型组比较MDA表达均明显减少(P<0.01),尤以TMP+针刺组改善明显(P<0.01)。各治疗组SOD活力均有提高,各组间差异无统计学意义(P>0.05)。结论:针灸配合TMP明显提高耳蜗组织内SOD活力,防止脂质过氧化,对急性耳毒性药物早期损伤具有一定的防治作用。

Objective：To investigate the effect of acupuncture and tetramethylpyrazine（TMP） on cochlear free radical expression of mice with acute drug-induced deafness. Methods：Fifty guinea pigs were randomly divided into 5 groups,including normal,model,TMP alone,acupuncture alone,and TMP plus acupuncture groups,10 animals in each group. Each group received different procedures. The normal group was prepared by consecutively intraperi- toneal injection with normal saline of 2.5 mL/（kg＇d）. Acute drug-induced deafness was made by consecutively in traperitoneal injection with gentamycin（lO0 mg/kg-d） in other groups. All groups were injected for consecutive 14 days. Before and 1 day after 14-day injection, the mice were tested ABR to identify whether the models were suc- cessful. The methods of thiobarbituric acid and xanthine oxidase were used to test serum malondialdehyde（MDA） and superoxide dismutase（T-SOD）. Results：The expression of MDA was reduced in all treatment groups as com- pared with that of the model group（P〈0.01）,with the most reduction seen in the TMP plus acupuncture group. The activity of SOD was enhanced in all treatment group,but with no significant difference among the treatment groups （P〉0.05）. Conclusion：TMP plus acupuncture can effectively increase the activity of SOD and reduce the expres- sion of MDA in the cochlear tissue. They may have effect against gentamycin-induced inner ear diseases.